以PCR技術快速鑑定鮪魚種類

[蕭仁傑]

我們發展出一種以分子技術來鑑定鮪魚種類。這種技術包括對於鮪魚魚種有專一性的探針以及引子,並以此來放大粒線體的基因片段。此方法可用於煮熟的鮪魚罐頭,新鮮樣本,或是經酒精保存過的樣本上,整個鑑定過程可於半天內完成。[Shiao JC]

We develop species-specific probe and primer for rapid and high-throughput identification of tuna species. The probe and primer are designed to amplify the mitochondrial genes. This assay can be applied to fresh, alcohol preserved and canned samples. The whole procedure can be conducted within half-a-working day.