1.0Institute of Oceanography, NTUhttps://www.oc.ntu.edu.tw/en/rich600338<blockquote class="wp-embedded-content" data-secret="lxrHXaV13x"><a href="https://www.oc.ntu.edu.tw/en/research-highlights/28373/">Rapid identification of tuna species by real-time PCR technique.</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://www.oc.ntu.edu.tw/en/research-highlights/28373/embed/#?secret=lxrHXaV13x" width="600" height="338" title="“Rapid identification of tuna species by real-time PCR technique.” — Institute of Oceanography, NTU" data-secret="lxrHXaV13x" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script> /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); </script> [Shiao JC] We develop species-specific probe and primer for rapid and high-throughput identification of tuna species. The probe and primer are designed to amplify the mitochondrial genes. This assay can be applied to fresh, alcohol preserved and canned samples. The whole procedure can be conducted within half-a-working day.https://www.oc.ntu.edu.tw/wp-content/uploads/2011/12/jay1.jpg